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. 2017 Sep;134:18–23. doi: 10.1016/j.brainresbull.2017.06.012

Fig. 2.

Fig. 2

Age-dependent impairment of αCaMKII activation in stratum radiatum of hippocampal area CA3 after contextual fear conditioning. A) Representative images of phosphorylated and total αCaMKII in the stratum radiatum (SR) and stratum pyramidal (SP) of the CA3 hippocampus. Total αCaMKII stained using dsRed (red), phosphorylated αCaMKII stained using eGFP (green). Young untrained (far left), young trained (centre left), aged untrained (centre right), aged trained (far right), scale 20 μm. B) Contextual fear conditioning does not alter total αCaMKII levels in the stratum radiatum of the CA3 hippocampus in young and aged mice. C) Contextual fear conditioning induced an increase in autophosphorylation of αCaMKII in young mice (p = 0.025), but there was no significant change in aged mice. D) Contextual fear conditioning induced an increase in the ratio of autophosphorylated to total αCaMKII in young (p = 0.012), but not aged mice. Ageing induced an increase in ratio of phosphorylated:total αCaMKII in untrained mice (p = 0.033). Mean ± standard error of mean, * (p < 0.05). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)