Table 1.
Primary and secondary antibodies used for immunohistochemistry staining for phosphorylated and total αCaMKII. Antibody concentrations, name and conditions for staining displayed.
| Experiment type | Antibody type | Name | Species | Special Conditions | Dilution |
|---|---|---|---|---|---|
| Phosphorylated αCaMKII (T286) | Primary | ABCAM, UK (AB5683) | Rabbit polyclonal | 18 h rt | 1:300 |
| Secondary | Life Tech, US (A11034) | Goat anti-Rabbit | 2 h rt | 1:2000 | |
| Total αCaMKII | Primary | Abnova Corp., ROC (MAB8699) | Mouse monoclonal | 72 h 4 °C | 1:2000 |
| Secondary | Life Tech, US (A11004) | Goat anti-mouse | 2 h rt | 1:500 |