Figure 5.

DEP-induced monocytes and lymphocytes are TNFR2 dependent. C57BL/6 J WT, TNFR1 KO and TNFR2 KO mice were exposed to saline (white bar) or 100 µg DEP (black bar). BALF was collected 48 hours after the last exposure. (A) Total cell numbers in BALF. (B–F) Neutrophil (CD11c^low, CD11b⁺, Ly6C⁺ and Ly6G⁺) (B); monocyte (CD11c^low, CD11b⁺, Ly6C⁺ and Ly6G⁻) (C); dendritic cell (CD11c^high, low autofluorescent and MHCII⁺) (D); CD4⁺ T-cell (CD3⁺, CD8⁻, CD4⁺) (E) and CD8⁺ T-cell (CD3⁺, CD4⁻, CD8⁺) (F) numbers in BALF were determined by flow cytometry. (G–I) CXCL1 (G), CCL2 (H) and CCL20 (I) levels were determined in BALF using ELISA. Results are expressed as mean ± SEM. n = 7–9 mice per group, except the TNFR2 KO mice receiving saline: n = 3 mice. *p < 0.05. BALF inflammatory cell data are representative of two independent experiments (except for BALF neutrophils).