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. 2017 Sep 14;7:11644. doi: 10.1038/s41598-017-11271-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Z-stack image series to analyse the phagocytic activity of apically applied monocyte-derived macrophages upon infection with A. fumigatus in co-culture with SAE cells. Co-cultures of SAE cells (white) grown under perfusion were transferred to static conditions and CFSE-labelled monocyte-derived macrophages (green) were apically applied prior to infection with A. fumigatus conidia (0.5 × 10⁶; MOI 0.5) for (a) 3 h, 6 h and (a) and (b) 24 h. (a) At 3 h (upper panel), single apically applied macrophages start migrating towards swollen conidia with single conidia already internalized into macrophages (arrow). At 6 h (middle panel) macrophage cluster around swollen/germinating conidia, while at 24 h (lower panel) many conidia were taken up by macrophages (arrows), which were also aggregating around hyphae on the SAE epithelium (b) x-y projections with respective side views are depicted in (a), while (b) shows 3D reconstructions at the 24 time point.