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. 2017 Sep 14;7:11527. doi: 10.1038/s41598-017-10078-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pseudo 3D migration of MCF7, MDA MB 231 and HT 1080 spheroid on the 2D surface. (A) A single spheroid is incubated on the glass coverslip and incubated for 24 hr. Reversal /melting of spheroids over time is analyzed by imaging with phase contrast mode of Nikon Eclipse Ti-U. The scale bar is 10 µm. (B) The covered area is calculated (NIS Elements software) and normalized with initial spheroid size for plotting (mean ± SEM).