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. 2017 Sep 14;7:11527. doi: 10.1038/s41598-017-10078-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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mRNA profiling of migration specific genes in 2D and 3D spheroid condition. (A) Representative image of semi-quantitative RT-PCR products of five migration specific genes (p130 Cas, Talin, Vinculin, Paxillin, Focal adhesion kinase) and β2 microglobulin on the agarose gel. (B) Densitometric analysis of PCR bands is done using ImageJ software. Statistical analysis (Student’s T test) is done and significant difference between groups (2D and 3D) is identified (* denotes p < 0.05).