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. 2017 Sep 14;7:11527. doi: 10.1038/s41598-017-10078-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Distribution of actin cytoskeleton and focal adhesion complex protein (vinculin) in 2D and 3D spheroid condition. Cells are grown in 2D (coverslip) and 3D (spheroid) conditions are fixed with 2% PFA and permeabilized with 0.01% Triton × 100. Staining is done with TRITC (red) conjugated phalloidin (actin); FITC (green) conjugated monoclonal anti-vinculin antibody (vinculin) and DAPI (blue) (nucleus). Imaging is done with 405, 488 and 653 nm Laser in Nikon AR1 confocal microscope using 60× oil objective. Spheroid imaging is done with optical Z scanning. The scale bar is 10 µm. Inset images in the lower panel are of a single spheroid in the 10× objective. Yellow arrows indicate vinculin distribution.