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. 2017 Sep 14;7:11587. doi: 10.1038/s41598-017-11886-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Removal of inhibitory domains from recombinant pro-antibodies by MMP-2. Unmodified anti-EGFR antibody (A), CBa-anti-EGFR antibody (B), C2b-anti-EGFR antibody (C) or LAP-anti-EGFR antibody (D) were digested by MMP-2 for the indicated time. Digested antibodies were resolved by reducing SDS-PAGE and analyzed by western blot using HRP-conjugated goat anti human-IgG Fcγ antibodies. Note that amounts of cut (mature form) antibodies increased as digestion was prolonged from 1 minute to 60 minutes. ID-HC: inhibitory domain-heavy chain. HC: heavy chain. with MMP: with MMP-2 incubation. The original blots image for panel A–D are presented in Supplementary Figure S5.