Figure 5.

The serum stability of LAP-anti-EGFR antibodies. (A) Anti-EGFR antibodies and LAP-anti-EGFR antibodies were incubated with 20% fetal bovine serum at 4 °C for indicated time, then analyzed for protein stability by western blotting using anti-human Fcγ secondary antibodies. Incubations of anti-EGFR antibodies or LAP-anti-EGFR antibodies with serum (FBS) did not cause hydrolyses of the antibodies, as compared to the non-treated (0 h) group. S: serum alone. (B) Anti-EGFR antibodies and LAP-anti-EGFR antibodies were incubated with 20% fetal bovine serum at 4 °C for indicated time, then analyzed for binding activity by cell-based ELISA. Serum incubation of anti-EGFR antibodies and LAP-anti-EGFR antibodies did not alter binding activity. The original blot image for panel A is presented in Supplementary Figure S7.