Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 14;7:11513. doi: 10.1038/s41598-017-11591-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Uncoupling of hCa_v3.2 from the calnexin C-tail increases surface expression of the channel. (A) Confocal images of living tsA-201 cells expressing hCa_v3.2-GFP channels (green, top left pannel) along with the mCherry-taged cytosolic domain of CNX (CNX^CD-mCherry) (red, top right panel). Overlaid images are also shown (bottom left panel). Note that in contrast to the CNX^BD-mCherry construct (Fig. 3B), the CNX^CD-mCherry construct that does not contain the ER transmembrane domain shows a diffuse expression pattern consistent with a soluble protein. (B) Representative Ba²⁺ current traces recorded from hCa_v3.2-HA- (left panel) and hCa_v3.2-HA/CNX^CD-GFP-expressing tsA-201 cells (right panel) in response to 150-ms depolarizing steps varied from −80 to +50 mV from a holding potential of −100 mV. (C) Corresponding mean current/voltage relationships for hCa_v3.2-HA- (black circles, n = 47) and hCa_v3.2-HA/CNX^CD-GFP-expressing cells (red circles, n = 48). The inset indicates the corresponding normalized maximum slope conductance G _max. Data are presented as mean ± SEM and were analyzed by Student’s unpaired t test; ***p < 0.001. (D) Low magnification confocal images of non-permeabilized (top panels) and permeabilized (bottom panels) tsA-201 cells expressing hCa_v3.2-HA alone (left panels) and together with calnexin C-tail (CNX^CD) (right panels) and stained for hCa_v3.2-HA (green) using a primary anti-HA antibody. (E) Corresponding mean surface and total expression of hCa_v3.2-HA channels expressed alone (black, n = 3) and in combination with CNX^CD (red, n = 3), assessed from non-permeabilized and permeabilized cells, respectively. (F) Internalization kinetics at 37 °C of hCa_v3.2-HA channels expressed alone (black, n = 3) and in combination with CNX^CD (red, n = 3). The inset indicates the time constant τ of internalization.