FIG 6.

Thermotaxis and cryophilic behavior depend on signals transmitted by PKC-2 in AFD neurons and intestinal cells. (A) Null mutations in genes encoding TRPA-1 and SGK-1 do not affect thermotaxis. (B) DN PKC-2B and CA PKC-2B transgenes were expressed in AFD neurons (gcy-8 promoter), intestinal cells (inx-16 promoter), or hypodermis (col-19 promoter) of WT animals. Thermotaxis assays were performed in triplicate as indicated in Materials and Methods; error bars are SEM. **, P < 0.01 compared to WT animals; ##, P < 0.001 compared to WT animals. Animals lacking EGL-3 have an AT phenotype.