Skip to main content
. 2017 Sep 12;37(19):e00040-17. doi: 10.1128/MCB.00040-17

FIG 4.

FIG 4

SEC is required for JQ1-mediated HBV induction. (A to C) JQ1 treatment slightly enhances the occupancies of SEC subunits CDK9, AFF4, and ELL2. (D) Knockdown of AFF4 in HepG2.2.15 cells by lentivirus-mediated shRNA targeting AFF4. (E) Knockdown of AFF4 in HepG2.2.15 cells inhibits basal and JQ1-induced HBV pgRNA transcription. Control and AFF4-depleted cells were treated with DMSO, for the vehicle control, and JQ1. The expression levels were normalized to the internal control GAPDH mRNA levels. Error bars represent the standard deviations from three independent measurements. (F) The CDK9 inhibitor flavopiridol inhibits basal and JQ1-induced HBV pgRNA transcription. pgRNA levels were measured by RT-qPCR and normalized to GAPDH mRNA levels. Error bars represent the standard deviations from three independent measurements. (G and H) BRD4 and AFF4 enrichment analysis in BRD4-depleted cells. The HepG2.2.15 cells were treated with BRD4- and AFF4-specific shRNAs for 2 days, followed by 48 h of JQ1 treatment. IgG serves as the nonrelated antibody control. The HEMO gene serves as a negative control for ChIP-qPCR. Error bars represent the standard deviations from three independent measurements.