TABLE 2.
Detection of PrPSc in lymphoid tissues by antigen capture EIA and RT-QuIC assay
| Treatment group and animal no. | Incubation period (mpi) | Overall result | RPLNb |
Tonsil |
MLNc |
|||
|---|---|---|---|---|---|---|---|---|
| EIA result | RT-QuIC result | EIA result | RT-QuIC result | EIA result | RT-QuIC result | |||
| Noninoculated controls | ||||||||
| 5 | 6 | − | − | − | − | − | − | − |
| 9 | 73 | − | NAa | NA | − | − | − | − |
| i.c. inoculated market wt | ||||||||
| 12 | 6 | + | − | − | − | − | − | + |
| 14 | 6 | + | − | + | − | + | − | − |
| 15 | 6 | + | − | + | − | + | − | + |
| 16 | 6 | + | − | + | − | + | − | + |
| 18 | 6 | + | − | − | − | − | − | + |
| 19 | 6 | − | − | − | − | − | − | − |
| i.c. inoculated aged | ||||||||
| 22 | 30 | + | − | − | − | − | − | + |
| 24 | 42 | + | NA | NA | − | + | NA | NA |
| 25 | 45 | + | − | + | − | − | − | − |
| 26 | 56 | + | − | + | − | + | NA | NA |
| 27 | 64 | − | NA | NA | − | − | NA | NA |
| 28 | 73 | + | NA | NA | − | + | − | + |
| 29 | 73 | + | − | + | − | − | − | − |
| Orally inoculated market wt | ||||||||
| 30 | 6 | + | − | − | − | − | − | + |
| 32 | 6 | + | − | − | − | + | − | + |
| 34 | 6 | − | − | − | − | − | − | − |
| 36 | 6 | + | − | − | − | + | − | + |
| 37 | 6 | + | − | + | − | − | + | + |
| 38 | 6 | + | − | − | − | + | − | + |
| Orally inoculated aged | ||||||||
| 39 | 19 | + | − | − | − | − | − | + |
| 41 | 41 | + | − | + | − | − | − | + |
| 43 | 45 | + | − | NA | − | + | NA | NA |
| 44 | 55 | + | NA | NA | − | − | − | + |
| 45 | 64 | − | − | NA | − | − | NA | NA |
| 48 | 72 | − | NA | NA | − | − | − | − |
a
NA, sample not available.
b
RPLN, retropharyngeal lymph node.
c
MLN, mesenteric lymph node.