FIG 1.

TLR9 transcription is induced by several cellular stresses in PHKs. (A) Total RNA and total proteins were extracted from UVB-irradiated (+) and mock-irradiated (−) PHKs; TLR9 expression levels were measured by RT-qPCR and normalized to GAPDH levels (left panel), and protein extracts were analyzed by immunoblotting for the indicated antibodies (right panel). (B and C) Total RNA and total proteins were extracted from PHKs treated (+) and mock treated (−) with 0.1 μg/ml doxorubicin (DOX) for 8 h (B) or with H₂O₂ (at the indicated concentrations) for 1 h (C). TLR9 expression levels were measured by RT-qPCR (left panels), and protein extracts were analyzed by immunoblotting for the indicated antibodies (right panels). The RT-qPCR data shown are the means from three independent experiments. The immunoblots shown are from one representative experiment of the three performed. *, P < 0.05; **, P < 0.01.