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. 2017 Sep 12;91(19):e01123-17. doi: 10.1128/JVI.01123-17

FIG 7.

FIG 7

ΔNp73α prevents recruitment of p53 to the TLR9 promoter. (A) Normal PHKs (control PHKs) and PHKs expressing HPV38 E6 and E7 (HPV38E6E7 PHKs) were UVB irradiated (+) or not irradiated (−). Total cellular extracts were processed for ChIP with the indicated antibodies. The error bars represent the standard deviations of three biological replicates. (B) PHKs expressing HPV38 E6 and E7 (HPV38E6E7 PHKs) were UVB irradiated (+) or not irradiated (−). Total cellular extracts were processed for ChIP with anti-p73 antibody (OP108; Calbiochem). The error bars represent the standard deviations of two biological replicates. (C) hTERT PHKs were transiently transfected with pCDNA HA-ΔNp73α and UV irradiated (+) or mock irradiated (−). After 8 h, total proteins were extracted, and ΔNp73α protein levels were determined by immunoblotting using HA-Tag antibody. (D) Cells treated as described in panel C were processed for ChIP experiments using p53 and HA-Tag antibodies. The error bars represent the standard deviations of three biological replicates. *, P < 0.05; **, P < 0.01; ***, P < 0.0001; ns, not significant.