FIG 8.

Role of TLR9 in cell viability of hTERT PHKs exposed to various doses of UV radiation. Cells were transfected with scramble or siTLR9. After 48 h, cells were collected for total RNA preparation to determine TLR9 mRNA levels (A) or cultured and exposed to the doses of UV radiation indicated (per cm²) (B). (A) TLR9 expression was determined by quantitative RT-PCR. (B) Cell viability after 4, 8, and 12 h of UV irradiation was determined using the CellTiter 96 AQueous One Solution cell proliferation assay (MTS). (C) Cell viability of hTERT PHKs and HPV38 E6/E7 hTERT PHKs after exposure to UV radiation. Cells were exposed to various doses of UV radiation (per cm²) as indicated in the figure. After 4 and 12 h, cell viability was determined as described in panel B. CTR, control (0 mJ/cm²).