TABLE 1.
Sequences of siRNA and CRISPR/Cas9 vectors used for gene silencing
| Target | Orientationa | siRNA sequence (5′–3′) or description (source) |
|---|---|---|
| Scrambled RNA (negative control) | GGUGGAAGAGGUGGUGAGC | |
| p65 | siGenome SMART pool M-003533-02-0005, human RELA, NM_021975 (Thermo Scientific) | |
| p53 | CAAUGGUUCACUGAAGACCUU | |
| p53 vector 1 | F | TCCATTGCTTGGGACGGCAAGTTTT |
| R | TTGCCGTCCCAAGCAATGGACGGTG | |
| p53 vector 2 | F | CCATTGTTCAATATCGTCCGGTTTT |
| R | CGGACGATATTGAACAATGGCGGTG | |
| p53 vector 3 | F | CTCGGATAAGATGCTGAGGAGTTTT |
| R | TCCTCAGCATCTTATCCGAGCGGTG | |
| p53 vector 4 | F | CACTTTTCGACATAGTGTGGGTTTT |
| R | CCACACTATGTCGAAAAGTGCGGTG | |
| Scrambled vector | F | GGATGGACGGTAGAGGTGGGTTTT |
| R | CCACCTCTACCGTCCATCCCGGTG |
a
Abbreviations: forward (F), reverse (R).