TABLE 2.
Sequences of primers used for RT-qPCR analyses, ChIP assays, and oligonucleotide pulldown assays
| Promoter or gene | Orientationa | Primer sequence (5′–3′) |
|---|---|---|
| TLR9 promoter NF-κB site C | F | GAGAGCACTCAGGGGAACAG |
| R | GGTCACATTCAGCCCCTAGA | |
| GAPDH | F | AAGGTGGTGAAGCAGGCGT |
| R | GAGGAGTGGGTGTCGCTGTT | |
| TLR9 | F | CGTCTTGAAGGCCTGGTGTTGA |
| R | CTGGAAGGCCTTGGTTTTAGTGA | |
| TLR3 | F | AACGACTGATGCTCCGAAG |
| R | TCCAGAGCCGTGCTAAGTTG | |
| TLR7 | F | TTGGCACCTCTCATGCTGTG |
| R | ACCATCTAGCCCCAAGGAGT | |
| p53 | F | GATGAAGCTCCCAGAATGCC |
| R | CAAGAAGCCCAGACGGAAAC | |
| p65 | F | GTCACCGGATTGAGGAGAAA |
| R | GCTCAGGGATGACGTAAAGG | |
| E6 | F | TCTGGACTCAAGAGGATTTTG |
| R | CACTTTAAACAATACTGACACC | |
| E7 | F | CAAGCTACTCTTCGTGATATAGTT |
| R | CAGGTGGGACACAGAAGCCTTAC | |
| Probes for oligonucleotide pulldown assays | F | Btn-GAGAGCACTCAGGGGAACAG |
| R | GGTCACATTCAGCCCCTAGA |
a
Abbreviations: forward (F), reverse (R).