FIG 5.

NCR1 plays an important role in reovirus-based treatment of B16 cells. (A) Tumor volumes of C57BL/6 Ncr1^+/+ (WT) and Ncr1^gfp/gfp (Ncr1 KO) mice 20 days after s.c. injection of 10⁴, 10⁵, or 10⁶ B16 cells. Statistically significant differences are indicated. *, P < 0.05; ns, not significant. (B) Percent survival of C57BL/6 Ncr1^+/+ (WT) and Ncr1^gfp/gfp (Ncr1 KO) mice following the injection of 5 × 10⁵ B16 cells. (C) Schematic representation of the experimental procedure used. Each reovirus injection consisted of 5 × 10⁹ PFU/mouse. I.T., intratumor. (D) Percent survival of the mouse groups (five mice per group) indicated in two independent experiments. Mice were euthanized when tumors reached a size of >1 cm³.