FIG 7.
NCR1 is essential for reovirus treatment in a model of lung carcinoma. (A) FACS staining of D122 cells incubated for 14 h in the presence (empty gray histogram) or absence (empty black histogram) of reovirus. Staining was performed with NKp46-Ig (A, B), NCR1-Ig (C, D), and NKG2D-Ig (E, F), as indicated on the x axes. The filled gray histograms depict the background staining of D122 cells with the secondary MAb only in the absence of reovirus. The background staining of D122 cells in the presence of reovirus was similar and is not shown. Shown are the results of one representative experiment out of three (NKp46-Ig) or five (NCR1-Ig and NKG2D-Ig) performed. The median fluorescence intensity (MFI) of the different repeats of NKp46-Ig (B), NCR1-Ig (D), and NKG2D (F) staining is shown. Error bars represent the standard error of the mean. Statistically significant differences are indicated. *, P < 0.05; ns, not significant. (G) Percent survival of C57BL/6 Ncr1+/gfp (Ncr1 Het) and Ncr1gfp/gfp (Ncr1 KO) mice treated with UV-inactivated reovirus or left untreated. Each UV-inactivated reovirus injection consisted of 2.5 × 1010 PFU per mouse. Empty circles represent untreated Het mice. Filled circles represent UV-inactivated-reovirus-treated Het mice. Empty squares represent untreated KO mice. Statistically significant differences are indicated in the graph. Shown are the results of one representative experiment out of two performed. In all flow cytometry experiments, antibodies and fusion proteins were incubated with target cells on ice. Fusion proteins were incubated for 2 h, and antibodies were incubated for 1 h.