US3-induced TNTs contain stabilized microtubules. TNTs induced in US3-transfected ST cells (A and B) or PRV-infected ST cells (C and D) contain acetylated (A and C) and detyrosinated (B and D) tubulin. (A) Magenta, acetylated tubulin; red, PRV US3; green, tubulin; cyan, nuclei. (B) Red, detyrosinated tubulin; magenta, US3; green, tubulin; cyan, nuclei. (C) Magenta, acetylated tubulin; red, PRV gD; green, tubulin; cyan, nuclei. (D) Red, detyrosinated tubulin; green, PRV gD; magenta, tubulin; cyan, nuclei. Bars, 30 μm. KD, kinase dead; WT, wild type; PRV, pseudorabies virus; ΔUS3, US3null mutant.