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Mutant M viruses elicit reduced transcriptional inhibition. EPC cells were left untreated and unstained (A), untreated and stained (B), treated with actinomycin D (C), or infected with the indicated viruses at an MOI of 1 for 24 h prior to 5-EU labeling of nascent RNA (D to F). 5-EU was conjugated to Alexa Fluor 594 by a Click-iT reaction and flow data were collected. The percentage of cells scored as AF-594 negative and AF-594 positive are shown as an inset within each histogram.
