FIG 3.

VHSV-IVb M inhibits host mRNA expression. (A) EPC cells (1 × 10⁶) were cotransfected with or without an SV40/luc construct (0.8 μg) and various concentrations of a VHSV-IVb M expression plasmid (0.1 to 0.5 μg) for 24 h, followed by RNA isolation. Luciferase mRNA levels were measured by RT-qPCR. Data were normalized to β-actin mRNA levels. (B) Tet-mSEAP was cotransfected or not cotransfected with IVb M (0.1 μg) into EPC cells for 24 h and then treated with doxycycline (Dox) for 24 h, followed by RNA isolation and RT-qPCR using the mSEAP primer. ****, P < 0.001.