FIG 2.

Localization of nascent viral DNA relative to the ER. A549 cells were infected with VACV at a multiplicity of infection of 10 PFU/cell. At 3.5 h after infection, the cells were incubated for 10 min with EdU and then fixed, permeabilized, and reacted with Alexa Fluor 488 azide. The cells were then incubated with rabbit polyclonal antibody to calnexin (Santa Cruz), followed by secondary antibody conjugated to Alexa Fluor 594 and DAPI. The image was made with Huygens deconvolution software and analyzed with Imaris 8.4.1.