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Kinetics of VACV DNA synthesis in the absence (−) and presence (+) of EdU. A549 cells were infected with VACV for 6 h in duplicate. At 3.5 h, EdU (10 μM) was added to one portion of the cells. At the time points shown, the cells were harvested, and the amounts of VACV DNA and host actin were determined by ddPCR.
