FIG 7.

Detection of I3 by Western blotting of proteins associated with affinity-purified EdU-biotin complexes. (A) Gel electrophoresis of deproteinized DNA from the 1% SDS-insoluble and -soluble fractions as shown in Fig. 6. (B) Proteins eluted from streptavidin beads were resolved by SDS-PAGE, electroblotted onto a nitrocellulose membrane, probed with a MAb to the VACV I3 protein, and detected by chemiluminescence. The control (−click) and experimental (+click) samples are indicated. The masses of marker proteins are shown on the right, with the position of monomeric I3 indicated by the arrow. The band of about 62 kDa is presumed to be an I3 dimer.