FIG 2.

B cell-specific depletion of ATM attenuates MHV68 latency at 16 days postinfection. (A and B) B-Cre-positive and -negative mice were infected intranasally with 10⁴ PFU of MHV68. At 16 days postinfection, splenocytes were harvested and subjected to limiting-dilution analyses to determine the frequency of ex vivo reactivation (A) and the frequency of infected cells (B). The dashed lines in these and other frequency experiments are drawn at 62.5%, and the intercept of the line with the data curve indicates the frequency of a positive event in the analyzed population. The frequencies of positive cells are indicated next to each genotype. Data were pooled from three independent experiments with 3 to 5 mice per group. The error bars indicate standard error of measurement. (C and D) The frequency (C) and absolute number (D) of B220⁺ splenocytes were determined in B-Cre-positive and -negative mice at 16 days following either mock or intranasal infection with 10⁴ PFU of MHV68. Each symbol represents an individual mouse; data were pooled from 3 independent experiments.