FIG 5.

EBNA1 forms dynamic higher-order complexes (HC) on 2FR DNA compared to the T585P mutant. (A) WT EBNA1 or the T585P mutant was titrated on DNA and allowed to form complexes for 30 min. On the right part of gel, the addition of identical but unlabeled 2FR DNA (740 nM) initiated the exchange of EBNA1 and the T585P mutant between the two sets of FR DNA molecules. (B) Quantification of total complexes (higher-order complex plus 2FR plus 1FR). (C) Quantification of higher-order complexes alone. (D) Quantification of all complexes as described above for panel B but in the presence of competitor DNA.