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. 2017 Sep 12;91(19):e00811-17. doi: 10.1128/JVI.00811-17

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Copyright © 2017 Bogers et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 9 — Env-specific cytokine-secreting T-cell immunosorbent spot assay responses following immunizations at 0, 4, 24, and 36 weeks (immunization phase) (A) and long-term 70 weeks follow-up (durability phase) (D). Shown are IL-4 (upper row), IL-2 (middle row), and IFN-γ (lower row) secreting spot-forming cells over time. Immunosorbent spot assays from individual animals immunized with gp140-CD4bs-x (occluded group; first column), gp140-CD4bs-open (second column), mini-CD4 mimetic only (third column), and adjuvant only (fourth column). Background responses (mean numbers of spots plus 2× the standard deviations from triplicate assays with medium alone) were subtracted. Responses after stimulation with overlapping SF162 gp120 20mer peptides are presented as the number of spot-forming cells per 10⁶ PBMCs. N.S., no significance.