FIG 1.

Structures of AIDSVAX B/E, HSV-1 gD, and amino acids of HSV-1 gD critical for binding of monoclonal antibodies CH42 and CH43. (A) AIDSVAX B/E has the first 27 amino acids of the mature form of HSV-1 gD fused to gp120 without the first 40 amino acids of gp120. (B) HSV-1 gD has a signal sequence and HVEM and nectin-1 binding domains. (C) HSV-1 gD amino acids recognized by MAb CH42 and CH43 were determined by binding the MAbs to peptides containing alanine substitution mutations in HSV gD (the amino acid sequence of HSV-1 gD TAG peptide is shown on the x axis), followed by ELISA. The optical density (OD) at 405 nm for binding of MAbs to gD TAG is shown on the y axes. Amino acid sequence alignment of HSV-1 gD TAG to HSV-1 and HSV-2 gD in five commonly used virus strains is shown below; the dashes represent amino acids identical to those in HSV gD TAG. The numbers indicate amino acid positions in the mature form of gD.