Figure 10.

Chromofungin (CHR) directly enhances migration, proliferation, and viability of colonic cell line. Caco-2 cells were pre-treated with 1% phosphate-buffered saline (PBS) or CHR (100 nmol/mL) or sCHR (100 nmol/mL) for 24 h, then challenged with lipopolysaccharide (LPS) (1 µg/mL) or 5% dextran sulfate sodium (DSS) for additional 24 h. (A,B) Epithelial cell migration assessed by the wound healing assay, (C) intestinal epithelial cell proliferation, (D) epithelial cell viability assessed by the 3-(4, 5-dimethyl thiazolyl-2yl)-2, 5-diphenyl tetrazolium (MTT) assay, and (E) epithelial cells oxidative stress assay shows survival data from cultures treated with normal medium (control) or 200 mmol/L H₂O₂. Two-way or one-way ANOVA was used to analyze the data followed by multiple comparison tests. Data represent mean ± SEM (n = 6). ^# refers to significance compared to control groups. Each experiment was repeated at least three times.