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. 2017 Sep 15;8:1131. doi: 10.3389/fimmu.2017.01131

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Copyright © 2017 Eissa, Hussein, Kermarrec, Grover, Metz-Boutigue, Bernstein and Ghia.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chromofungin (CHR) enhances the polarization of alternatively activated macrophages (AAM) in vitro. Peritoneal macrophages collected from naive C57BL6 mice and pretreated with CHR (200 ng/mL) for 2 h then stimulated by interleukin (IL)-4/IL-13 (20 ng/mL) for 6 h. (A) Protein levels of IL-10 and arginase activity and (B) mRNA levels of AAM markers [Il10, arginase (Arg1), Ym1 chitinase-like protein (Ym1), and found in inflammatory zone protein (Fizz1)]. One-way ANOVA followed by multiple comparison tests. Each value represents the mean ± SEM, n = 3–5/group. ^# refers to significance compared to control groups, Each experiment repeated at least three times.