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. 2017 Sep 15;8:1131. doi: 10.3389/fimmu.2017.01131

Figure 7.

Figure 7

Chromofungin (CHR) indirectly maintains gene expression of tight junction (TJ) proteins and decreases interleukin (IL)-8 and IL-18 release from lipopolysaccharide (LPS)- and dextran sulfate sodium (DSS)-stimulated colonic cell line through alternatively activated macrophages (AAM) conditioned medium. Peritoneal macrophages collected from naive C57BL6 mice and pretreated with CHR (200 ng/mL) for 2 h then stimulated by IL-4/IL-13 (20 ng/mL) for 6 h. Caco-2 cells were cultured in 2 mL supernatants of 1% phosphate-buffered saline (PBS) or CHR (100 nmol/mL) or sCHR (100 nmol/mL) treated AAM conditioned medium for 24 h, then challenged with LPS (1 µg/mL) or 5% DSS for 24 h. Cells and supernatants harvested for analysis. (A) IL-8 and IL-18. (B) Colonic mRNA levels of TJ proteins [claudin-1 (CLDN1), zonula occludens-1 (ZO1), E-cadherin (CDH1), occludin (OCLN)]. One-way ANOVA followed by multiple comparison tests. Data represent mean ± SEM (n = 6). # refers to significance compared to control groups. Each experiment repeated at least three times.