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Enhancement by PLD activator of enzymatic conversion of GM2 to GM3 ganglioside catalyzed by β-hexosaminidase A. Purified PLD activator was loaded on a Superdex 200 column (Fig. 1). Each fraction was assayed for the ability to stimulate enzymatic conversion of GM2 to GM3 ganglioside catalyzed by β-hexosaminidase A. PLD activation also is plotted in the same figure. •, PLD activity; ○, GM3 formation.
