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(A) SDS-PAGE gels stained with coomassie of bead-milled biomass, under reducing conditions and corresponding Western Blot, detecting rabbit polyclonal antibodies against the large subunit of Rubisco with polyclonal goat antirabbit immunoglobulins (B). (C) SDS-PAGE gels stained with coomassie of ASPIs (C). M = molecular weight marker, T = T. impellucida, A = A. maxima, S = S. dimorphus, and N = N. gaditana.
