Figure 46.

Stereoview of the simulated annealing omit map of 2-fluoroneryl diphosphate (2FNPP) bound in the active site of methylisoborneol synthase. Only two metal ions bind in this complex; metal coordination interactions are indicated by red dashed lines. This complex was obtained by cocrystallizing the enzyme with racemic 2-fluorolinalyl diphosphate, indicating that fluorination does not completely hinder ionization and formation of this catalytic intermediate. Reproduced from ref (213). Copyright 2013 American Chemical Society.