Figure 54.

(A) Biosynthetic manifold of wild-type and mutant epi-isozizaene synthase enzymes. The predominant cyclization product of the wild-type enzyme, epi-isozizaene, is highlighted in a cyan box, and the predominant cyclization products of mutant enzymes are highlighted in blue boxes. Side products are highlighted in yellow boxes. Reproduced from ref (244). Copyright 2014 American Chemical Society. (B) F95H epi-isozizaene synthase (EIZS) generates β-curcumene with 68-fold enhanced catalytic efficiency compared with the generation of α-bisabolene by wild-type α-bisabolene synthase, thereby providing a more efficient route for the generation of the D2 diesel fuel substitute bisabolane.