Fig 5.

Analysis of autophagy flux by subcellular colocalization. (A) Combination of autophagosomes with p62-cargos was disrupted by Palmitic acid treatment. Decrease of co-localized punctas of LC3B-P62 analyzed by Pearson' correlation coefficients, the distribution and structure of autophagosomes (LC3B) and p62 were changed as rod-like shape and departing from the nuclei, separately, in Palmitic acid-treated cells. White arrows indicate conjunction of LC3B with p62-cargos; pink arrows indicate alone p62-cargo aggregates in rod-like shape. (B) Conjugation of lysosomes with p62 was decreased by Palmitic acid treatment. The marginalized distribution and abnormal structure of lysosomes were associated with the irregular p62-cargo, and more unconjuaged p62-cargos situated at the internal cytoplasm in Palmitic acid-treated cells. The open boxes in the overlay pictures are amplified at the right columns. Scale bar = 10 µm. Pearson' correlation coefficient analysis was based on 10 sight fields each group. *p<0.05, **p<0.01 vs control; ## p< 0.01 vs 250 μM PA group.