Fig 6.

Intracellular insulin was involved in formation of Autophagolysosomes. (A) Formation of Autophagolysosomes was suppressed in Palmitic acid-treated cells. The fusion of lysosomes with autophagosomes was reduced and marginalized, and more aggregates of lysosomes (the red structures) or p62 (the green structures) were separately distributed in PA-treated cells. (B) The association of preproinsulin with p62 was marginalized at the cellular periphery; and some preproinsulin aggregates (the red structures) lonely accumulated in the intermediate area in PA-treated cells. The open boxes in the overlay pictures are amplified at the right columns. Scale bar = 10 µm. Pearson' correlation coefficient analysis was based on 10 sight fields each group. *p<0.05 vs control.