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. 2017 Jul 18;13(8):996–1007. doi: 10.7150/ijbs.19986

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Osthole increases cartilage and bone volume of fracture callus. (A) Histological analysis showed that Osthole accelerated the fracture healing process. (B) The Alcian blue staining positive cartilage area of callus tissues, (%, Cg.Ar/Ps.Cl.Ar) was increased after Osthole treatment at day 7 and slightly reduced at day 10. (C) New woven bone formation in callus tissues (%, Md.Ar/Ps.Cl.Ar) was enhanced by Osthole treatment at day 10-21. Black arrows: cartilage area; blue arrows: woven bone area. Data are presented as means ± SD. *P < 0.05, n=10.