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. 2017 Jul 18;13(8):996–1007. doi: 10.7150/ijbs.19986

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The effects of Osthole on the increases in cartilage and bone volume in fracture callus were inhibited Bmp2^Col2Cre KO mice. (A) Histological analysis showed that Osthole could not accelerate the fracture healing process for Bmp2^Col2Cre group. (B) The Alcian blue staining positive cartilage area of callus tissues (%, Cg.Ar/Ps.Cl.Ar) was lower in Bmp2^Col2Cre group compared with Cre-negative group after Osthole treatment at day 7. (C) New woven bone formation in callus tissues (%, Md.Ar/Ps.Cl.Ar) was lower in Bmp2^Col2Cre group compared with Cre-negative group after Osthole treatment at day 10. Black arrows: cartilage area; blue arrows: woven bone area. Data are presented as means ± SD. *P < 0.05, n=10.