Dynamic expression of Wnt read-out genes (β-Catenin, Axin2, Lef1, Tcf4) during morphogenesis of DM3. (A-L') In situ hybridization of β-Catenin, Axin2, Lef1 and Tcf4 during tooth germ morphogenesis; Boxed regions in C,F,I,L were magnified in C',F',I',L'. (A) β-Catenin was expressed both in epithelium and mesenchyme at E40. (B) β-Catenin was mainly located in the epithelium at E50. (C,C') At E60, β-Catenin was mainly expressed in the lower parts of IEE (red arrows) rather than the tip of the cone. (D) Axin2 was expressed in the epithelium and the beneath mesenchyme region at E40. (E) Axin2 was mainly expressed in the enamel knots and the underlying mesenchyme regions at E50. (F,F') At E60, Axin2 was expressed mainly in the lower parts of IEE. (G-H) Lef1 was expressed in enamel knots and the underlying mesenchyme at E40-E50. (I,I') At E60, Lef1 was expressed in lower parts of IEE and pre-odontoblasts (yellow arrows). (J) Tcf4 was mainly expressed at the mesenchyme region at E40. (K) At E50, Tcf4 was expressed both in epithelium and dental papilla. (L,L') Tcf4 was expressed in IEE, dental papilla and odontoblasts at E60. Scale bars represent 50μm (A,D,G,J), 100μm (B,C',E,F',H,I',K,L') and 200μm (C,F,I,L).