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VP inhibited the proliferation of NB4 cells. (A-B) Cell viability was detected by CCK-8 assay. (C) The in vitro colony-forming ability of NB4 cells was tested. (D-E) Cells cycle distribution was tested by FCM. (F-H) Effects of VP on protein expression levels of c-Myc and cyclinD1 were determined by western blot. Quantitative analysis was performed by measuring the relative expression levels of c-Myc and cyclinD1 to that of β-Actin. Data are expressed as means ± SD. *P < 0.05.
