Figure 2.

VP induced apoptosis of NB4 cells. (A-B) Cells were treated with VP for 24 h, and apoptosis was analyzed by FCM. (C) Apoptotic cells were observed by Hoechst 33342 staining (Magnifications 20x). (D-J) The effects of VP on the protein expression levels of PARP, cleaved PARP, Bcl-2, Bax, caspase3, and cleaved caspase3 were determined by western blot. Quantitative analysis was performed by measuring the relative proteins expression level to that of β-Actin. Data are expressed as means ± SD. *P < 0.05.