Table III.
Total fatty acid (TFA), EPA content (% dry biomass; mean values ± SD), and fatty acid composition (% of TFA) of Phaeodactylum tricornutum UTEX 640 cultivated in 40‐L GWP®‐III reactors under different nitrogen supply regimes in summer and spring
| TFA a (% dry biomass) | EPA (% dry biomass) | Sat (% TFA) | Mon (% TFA) | PUFA b (% TFA) | EPA (% TFA) | |
|---|---|---|---|---|---|---|
| Summer 6 days | ||||||
| NR | 7.17 ± 0.25 | 2.26 ± 0.16 | 20.92 | 17.29 | 61.92 | 31.52 |
| L3 | 10.68 ± 0.49 | 2.81 ± 0.31 | 24.91 | 25.75 | 49.34 | 26.31 |
| S | 20.91 ± 2.62 | 1.83 ± 0.38 | 39.93 | 43.95 | 16.21 | 8.75 |
| Summer 8 days | ||||||
| NR | 7.11 ± 0.66 | 2.06 ± 0.28 | 23.21 | 20.82 | 55.84 | 36.57 |
| L3 | 11.65 ± 1.33 | 1.98 ± 0.09 | 29.87 | 31.76 | 38.37 | 17.00 |
| S | 24.58 ± 1.07 | 1.78 ± 0.00 | 41.58 | 45.28 | 13.18 | 7.24 |
| Spring 6 days | ||||||
| NR | 7.05 ± 0.67 | 2.38 ± 0.24 | 22.13 | 18.72 | 59.00 | 33.76 |
| L3 | 12.65 ± 0.21 | 2.60 ± 0.17 | 32.25 | 31.78 | 35.97 | 20.55 |
| L2 | 15.24 ± 1.11 | 2.66 ± 0.30 | 34.91 | 34.19 | 30.38 | 17.45 |
| L1 | 18.48 ± 2.02 | 2.42 ± 0.40 | 39.39 | 36.04 | 24.03 | 13.09 |
| S | 25.63 ± 1.54 | 2.67 ± 0.23 | 41.47 | 40.11 | 18.03 | 10.42 |
NR = nitrogen‐replete culture; L1, L2, L3 = limited cultures with nitrogen supplied once, twice, and thrice per day; S = starved culture; Sat = saturated FA; Mon = monounsaturated FA; PUFA = polyunsaturated FA; EPA = eicosapentaenoic acid. Data refer to the last day of the period. In the summer trial, analyses were done both after 6 and 8 days.
a
TFA content at the beginning of the trial was about 7% on dry biomass both in summer and spring trials.
b
Including EPA.