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. 2017 Jul 14;91(4):565–573. doi: 10.1111/tpj.13601

Figure 1.

Figure 1

Structure of the CRISPR–dCas9 construct. (a) Transcription of Sp/Sa‐dCas9 was initiated by the parsley ubiquitin 4 promoter and terminated by the pea 3A terminator. An SV40 NLS DNA sequence was used for nuclear localization of dCas9. Transcription of the sgRNA scaffold was initiated by the Arabidopsis ubiquitin 6 promoter. (b) Protospacer design for Sp‐dCas9 and Sa‐dCas9 to target telomere DNA sequence. Target sequence is shown in red. The NGG protospacer adjacent motif (PAM) for Sp‐dCas9 is indicated in blue, whereas NNGRRT PAM for Sa‐dCas9 is indicated in green.