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. 2017 Jul 3;114(10):2328–2338. doi: 10.1002/bit.26346

Figure 1.

Figure 1

Schematic overview of strategies for the generation of linear DNA templates. (A) Amplification of the target gene was initially realized using gene‐specific and IRES‐specific primers, respectively (red). Adapter primers (blue) were applied in a second PCR step to link the amplified target gene to regulatory sequences, signal sequences and sequences encoding affinity tags. (B) Sequence of adapter primers, genespecific, and IRES‐specific primers. Overlapping sequences are underlined and the spacer (S) sequence is highlighted in orange. (C) Schematic overview of the generated linear DNA templates harboring regulatory elements.