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. 2017 Jul 3;114(10):2328–2338. doi: 10.1002/bit.26346

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© 2017 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Synthesis of different GPCRs in Sf21 cell lysates. Cell‐free protein synthesis was performed in the presence of 14C‐leucine. (A) Qualitative analysis of cell‐free synthesized GPCRs by SDS‐PAGE and autoradiography. MOR, µ opioid receptor; GRM1, metabotropic glutamate receptor 1; GLP1R, glucagon‐like peptide 1 receptor; GPR56, G‐protein coupled receptor 56; TSHR,thyrotrophic receptor; CXCR4, C‐X‐C chemokine receptor type 4; CXCR5, C‐X‐C chemokine receptor type 5. (B) Quantitative analysis of ¹⁴C labeled receptors by liquid scintillation counting.