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. 2017 Jul 3;114(10):2328–2338. doi: 10.1002/bit.26346

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© 2017 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Fluorescence analysis of eYFP/mCherry‐tagged receptors synthesized in the coupled Sf21 cell‐free system using CLSM (LSM 510 Meta, Carl Zeiss). Fluorescent insect vesicles in the vesicular fraction (VF) indicate efficient incorporation of MOR into microsomal membranes. NTC: no template control. Samples were excited at 488 nm using an argon laser and fluorescence emission was recorded with a longpass filter in the wavelength range above 505 nm. Arrows exemplarily indicate a microsomal vesicle harboring the fluorescent target protein.