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. 2017 Jul 3;114(10):2328–2338. doi: 10.1002/bit.26346

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© 2017 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Characterization of the binding properties of [3H] DAMGO and [³H]‐Naloxone (NLX) to MOR expressed in HEK 293 cells and MOR expressed in a cell‐free synthesis system. (A) [³H]‐DAMGO saturation binding experiments of HEK MOR and TCF‐MOR in presence of different detergents. (B and C) [³H]‐NLX saturation binding experiments of HEK MOR and TCF‐MOR in the presence of different detergents. (D) [³H]‐DAMGO single point binding experiment of HEK MOR, TCF‐MOR, NCF‐MOR, and NCMF‐MOR (n = 6) presented as counts per minute (cpm). (E) [³H]‐DAMGO single point binding experiment of HEK MOR, TCF‐MOR, NCFMOR, and NCMF‐MOR (n = 6) presented as fmol/mg total protein.